Surveillance of viral diseases in free-living turbot (Scophthalmus maeoticus, Pallas, 1814) in the Northern Black Sea and Sea of Azov

Purpose. The development of marine aquaculture in the Black Sea is relevant and promising for many reasons, as stated decisions documents national organizations region General Fisheries Commission Mediterranean (GFCM). Turbot one most valuable fish marketed seaside countries well European Union. Various aspects biology turbot have been investigated yet.Butunfortunately, surveillance procedures listed diseasesandanalysis possibility its prevention spread not provided. Therefore, this study, order to evaluate data on viral diseases Ukraine, 2020 a field survey free-living (Scophthalmus maeoticus) northern parts Azov was carried out. Methodology. Before sampling all fish were observed external lesions, measured from snout tail length total weight. Sampling included pooled internal organs, gills brain. Cell culture polymerase chain reaction (PCR) methods used identify diseases. Findings. An examination did reveal any sign disease. gills, good condition had appropriate color, shape without pathological changes. All tested samples free IPNV, VHSV VNN viruses determined by cell PCR. cytopathic effect (CPE) cells after first second blind passages. Using RT-PCR method, we looking for. Originality. This attempt screen Ukrainian waters Seas. Practical value. In future permanent turbotin accordance with EU strategy animal health allows prevent outbreaks develop new approaches diagnostic tests purpose study ecology pathogens different areas. Key words: turbot, Sea, diseases, culture,